Document Type : Primary Research paper
Assistant Professor, Pharmacognosy Lab, Dept. of Animal Health and ManagementStudies, Alagappa University, Karaikudi, Tamil Nadu, India.
Background : Cow distillate, popularly known as Cow ark, reportedly contains several therapeutic biomolecules and used as a remedy for diverse ailments including chronic and acute diseases. Hepatocellular carcinoma (HCC) is an aggressive disorder, causing significant cancer associated mortality worldwide.
Aim: In the present investigation, efficacy of cow ark on antioxidant ability and anticancer actions on HCC were determined and reported.
Methodology: Indigenous cow ark has been characterized and obtained aqueous fractions were validated using, series of assays. Investigation was performed on cow ark antioxidant ability by adopting DPPH radical scavenging and Superoxide measures, and results have shown to be remarkable effects, compared with prior reports. Findings favour the claim of Indian medicine health providers. Consequently, biochemical constituents of cow ark such as urea, phenol and amino acids were determined, and presented. Moreover, effects of cow ark on HCC was examined, using intra peritoneal administration of diethyl nitrosamine (DEN) to experimentally induce HCC (200 mg /kg b.w), with corn oil as vehicle in the wistar rats model. The hepatocytes were separated using collagenase perfusion measure and all tumor hepatocytes were segregated using flow cytometry. The effects of cow ark, as single, and enhancing plant extract (PE), and combined effects were analysed.
Results: Our study results exhibited that cow ark has the potential to accelerate Reactive Oxygen Species production ,and allow to increase membrane permeability (MP) and efficient discharge of Cytochrome c in HCC cancer cells while, no remarkable change was recorded in control hepatocytes. Conclusion: Our findings collectively endorsed that cow ark is a promising and novel therapeutic alternate and supported evident to the emerging cancer research in India.