Molecular Characterization And Phylogenetic Analysis Of The Pathogen Lasiodiplodia Pseudotheobromae Causing Black Leaf Spot Disease On Croton Bonplandianum(L), Plant.

Document Type : Primary Research paper

Authors

1 PhD scholar, Department of Botany, Andhra University, Visakhapatnam, Andhra Pradesh, India.

2 Professors, Department of Botany, Andhra University, Visakhapatnam, Andhra Pradesh, India.

Abstract

Lasiodiplodia pseudotheobromae is an important weed pathogen of Croton bonplandianum in the red gram crop fields. The aim of the present study was to molecular characterization and phylogenetic analysis of the fungal pathogen Lasiodiplodia pseudotheobromae .In this we will demonstrate that the DNA barcoding is the major tool for molecular analysis and Lasiodiplodia pseudotheobromae is represented by phylogentic tree for provides the systematic frame work for future analysis. The best phylogeny of the pathogen was given with the help of maximum parsimony (MP), maximum likelihood (ML), and neighbour joining (NJ) methods. As for the best of my knowledge the Lasiodiplodia pseudotheobromaeis the first reported in Croton bonplandium weed plant in Vinukondal mandal of Guntur district

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