Resurgence Of Multiple Shoots From Photosynthetic Explant Of Red Listed Lindernia Antipoda (L.) Alston

Document Type : Primary Research paper


PG and Research Department of Botany, Government Arts College (Autonomous), Thanthonimalai, Karur-639 005. Affiliated to Bharathidasan University, Tiruchirappalli – 620 024, Tamil Nadu, India.


Lindernia antipoda (L.) Alston a valuable ornamental wetland plant of Scrophulariaceae family. The in vitro studies on shoot induction and flowering in half-strength MS basal medium free of PGRs supported superior plantlets from photosynthetic explants (leaf). A series of modifications before complete shootlets were observed and the data were analyzed, interpreted, and recorded at an interval of 15 days. The explants produced an average of 99.36±1.51 shoots with 5.07±0.29 cm length having 34.68±1.71 number of roots of length 4.07±0.16 cm. The plantlets gave in vitro flowers and fruit that were identical to the in vivo plant. The well-developed plantlets were hardened and acclimatized. Plants devoid of required amount of nutrients and growth regulators can encounter difficulties but in the present investigation the selected species marked a profound growth imparting the essentialities are available remarkably in plants by nature itself. In future a modified growth medium can be attained with no doubts. The current study aims in PGR free plantlet regeneration in half strength MS basal medium.