Antimicrobial And Anticancer Activity Of Protein Hydrolysate And Crude Extract Of Snail Clithon Oualaniense (Lesson 1831)

Document Type : Primary Research paper

Authors

1 Department of Zoology, Vivekananda college, Agasteeswaram, Tamil Nadu, India

2 Department of Zoology, Holy Cross College (Autonomous), Nagercoil, Tamil Nadu, India Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli – 627 012, Tamil Nadu, India.

3 Department of Biomedical Science, Alagappa University, Karaikudi, Tamil Nadu, India.

Abstract

Clithon oualaniense is a brackish water snail with an operculum. It is an aquatic gastropod Mollusca with variable colouration pattern and small in size. Many bioactive compounds have been investigated for their antimicrobial, cytotoxic, anti-tumor and anti-inflammatory, and antiviral properties of mollusks. The present study is to investigate the antimicrobial activity and anticancer activity of protein hydrolysate and crude extract of snail Clithon oualaniense (Lesson 1831). Protein hydrolysate was prepared from tissue of Clithon oualaniense by enzymatic hydrolysis. 2.9 mg/ml protein concentration was estimated by Bradford’s method and 20 to 235 kDa protein bands were found in SDS PAGE analysis. Antibacterial assay was carried out against six bacterial pathogens by agar disc diffusion method. In antibacterial activity, the maximum zone of inhibition was observed against Staphylococcus aureus (23 mm) at 100μg/ml concentration of crude extract and 20mm at 100μg/ml concentration of protein hydrolysate. This study showed that the snail Clithon oualaniense crude extract and protein hydrolysate could be used as an antibiotic in biomedical research.

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