Adil Abdullah Wani author Malvika Sikdar-Bar author text article 2014 eng This investigation was intended to test the possible protective role of taurine and garlic extract against the toxicity of copper sulphate to Clarias gariepinus. For this purpose experiment was setup in seven groups (I-VII) containing 20 fish in each group. The fish of group I were kept as control. The fish of groups of II, IV and VI were challenged with 4 ppm solution of copper sulphate, where as groups III, V and VII were exposed to 8 ppm copper sulphate. Simultaneously, groups II and III were maintained as copper sulphate exposed non antioxidant treated control whereas, groups IV and V were treated with taurine (5 ppm) and groups VI and VII were treated with garlic extract (5 ppm) during the entire experiment period of 90 days. Histopathological observation of the gills after 15, 30, 60 and 90 days on exposure of sublethal copper sulphate concentrations revealed severe histopathological changes including, lamellar epithelium lifting, disintegration in pillar cell system with formation of aneurysms, increased infiltration of erythrocytes and leucocytes, haemolysis and haemorrhage, hyperplasia, complete fusion of secondary lamellae. While as addition of garlic extract and taurine has comparatively minimized histopathological alterations in groups VI and VII, respectively. It seems that simultaneous exposure of fish to taurine or garlic extract with copper sulphate were found to partly mitigate its toxicity indicating their potential therapeutic activity against copper toxicity in fish. 2008-8019 5 v. 2 no. 2014 113 129 http://www.journal-aquaticscience.com/article_73456_4e61ca83f50359e525033b88780c504f.pdf María José Sánchez-Muros author Cristina Elena Trenzado Romero author Miriam Furné Castillo author Fernando García Barroso author Ana Sanz Rus author text article 2014 eng This study investigated effect of repeated exposure to low doses of diuron in gilthead sea bream by measuring the activities of the following antioxidant enzymes: Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione transferase (GST), and DT-diaphorase (DTD). Examination of the lipid peroxidation (malondialdehyde content- MDA) and glucose 6-phosphate dehydrogenase (G6PDH) activity in these fish was also done. The results show that diuron exposure induces effects that are evident after 15 days of treatment. Almost all tissues are affected by treatment with diuron. MDA value of muscle and liver can be used as a biomarker of alterations in the oxidative state and therefore of the health of the fish. 2008-8019 5 v. 2 no. 2014 130 144 http://www.journal-aquaticscience.com/article_73461_2b7985ed9a9fb6c3da329a9d7c50e6fb.pdf Dung The Vo author Cong Van Chu author Dung Thi Vo author Glenn Allan Bristow author text article 2014 eng This article reports a survey of parasites in wild glass eels following them through culture to elvers in over-flow and re-circulating water systems in Vietnam. The results show that wild glass eels are infected with three parasitic species (Trichodina sp., Ichthyophthirius multifilis and Pseudodactylogyrus anguillae). Eels in re-circulating systems are infected with three parasites (Trichodina acuta, Trichodina sp., and Pseudodactylogyrus anguillae). Seven parasitic species were found on/in eels in the over-flow system (T. acuta, Trichodina sp., I.multifilis, P. anguillae, Pseudodactylogyrus bini, Centrocestus formosanus (metacercaria), and, Haplorchis taichui (metacercaria). Two parasites, Trichodina sp., and P. anguillae, were found on wild glass eels and elvers in both rearing systems. T. acuta had the highest prevalence (57.3%) in the recirculating system. P. bini had the highest prevalence (56.1%) in the over-flow system. The prevalence of Trichodina sp. infected fish in the recirculating system was 39.6%. That of C. formosanus (metacercaria) was 10.6% in the over-flow system. The prevalence of other parasites in all habitats was less than 10.0%. Two out of 3 parasites found in the re-circulating system had higher prevalence than those in the over-flow system. Results show that elvers reared in over-flow water systems had more parasites than wild glass eels or those reared in recirculating water system. 2008-8019 5 v. 2 no. 2014 145 153 http://www.journal-aquaticscience.com/article_73468_70e70dc03078464210bc3b360fe02df3.pdf María Florencia Tano de la Hoz author Alicia Mabel García author Mariano González Castro author Alcira Ofelia Díaz author text article 2014 eng Juveniles of Odontesthes argentinensis were collected from Mar Chiquita coastal lagoon, Argentina. The morphology of the gills was analyzed by scanning electron microscopy. The surface of the filaments and the pharyngeal region of the gill arch were covered by a mosaic of polygonal epithelial cells with apical concentric microridges. The apical crypts of mitochondria-rich cells were mainly found in the trailing edge of the filament epithelium and in the interlamellar surfaces. Glycoconjugates (GCs) elaborated by the secretory cells in the epithelium covering the gill filaments and the pharyngeal region of O. argentinensis were studied by means of a series of carbohydrate histochemical methods. Mucous cells among the lining epithelium of the pharynx showed a histochemical profile similar to that of mucous cells of filaments and secondary lamellae. Mucous cells showed the presence of neutral, sulphated, carboxylated and sialylated GCs. Glycoconjugates secreted on the surface of the gills could be associated with different functions such as lubrication, ionic regulation and inhibition of pathogen proliferation. 2008-8019 5 v. 2 no. 2014 154 166 http://www.journal-aquaticscience.com/article_73485_feb3de38d6e1af6704b956f93bbb1f8e.pdf Sakineh Yeganeh author text article 2014 eng In the present study, fatty acid composition was determined in eyed egg embryo and larval development stages in Rainbow trout (Oncorhynchus mykiss). All fatty acids revealed significant differences between eyed egg and 10-days larvae (p<0.05) except for docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA), eicosapentaenoic acid (EPA)/docosahexaenoic acid (DHA), total unsaturated fatty acids (∑ USFA)/ sum of unsaturated fatty acids (∑ SFA). However, in both larvae before (19, 22, 24, 27, 29) and after complete absorption of the yolk sac (34, 39, 44, 49, 58), there was an apparent preference in the utilization of polyunsaturated fatty acids (PUFA) followed by monounsaturated fatty acids (MUFA). Palmitic acid (C16:0), oleic acid (C18: 1n-9) and DHA (C22: 6n-3) were the main SFA, MUFA and PUFA, respectively, which were utilized and significantly decreased (p< 0.01) from 12-day larvae (day 19, with 80% yolk sac) to 22-day larvae as well as from 27-day larvae to 51-day larvae and then reached to the minimum levels in 22 and 51-day larvae. In larvae, these 3 nutrients were also the most fatty acids utilizing as energy source and possibly as precursors for others monounsaturated fatty acids biosynthesis as well. During the same period and among (n-6) polyunsaturated fatty acids, linoleic (18:2n-6); LA) and arachidonic (20:4n-6); AA) acids contents significantly decreased (p< 0:05) from 12-day larvae to 22-day larvae as well as from 27-day larvae to 51-day larvae. DHA is generally spared for physiological functions. 2008-8019 5 v. 2 no. 2014 167 180 http://www.journal-aquaticscience.com/article_73387_0b355adc2029f7cd3d87556e84962959.pdf Elagba Haj Ali Mohamed author text article 2014 eng Morphological revision was made on thirty specimens of Ichthyborus besse besse, and thirty specimens of Ichthyborus besse congolensis,taken from the collection of the Royal Museum for Central Africa in Belgium. The aim of this study is to verify the taxonomic status of the two subspecies and determine the morphological characters that characterize the ecological populations of I. besse besse from Cameron, Central Africa, and Congo and Schad basins, in their natural range. Variations of 23 morphometric measurements and 10 meristic counts were examined and compared. Principal component analysis along with discriminate analysis indicated that the most important morphometric characters that distinguish I. besse besse from I. besse congolensis are the snout length (SNL), body depth (BD), the origin of the pelvic (PRPV), anal (PRAN) and pectoral (PRP) fins, caudal peduncle length, in addition the number of: teeth in the upper jaw (TUJ) and the lower jaw (TLJ), scales around the caudal peduncle (CPS), number of scales in the lateral line (LLS). Cluster analysis of morphometric measurements produced a hierarchical cluster separating all individuals of I. besse besse from individuals of I. besse congolensis. Discriminate analysis of the ecological populations of I. besse besse revealed that the caudal fin length (CFL) and body depth (BD) have the bigger loads in separating the ecological populations followed by the inter-orbital width (IOW), eye diameter (ED), dorsal-to-adipose distance (DAD) and snout length (SNL), in addition to the number of teeth in lower jaw (TLJ) and upper jaw (TUJ). Cluster analysis of meristic counts gave better separation of the ecological population of I. besse besse than the morphometric measurements. A detailed description of I. besse besse and I. besse congolensis according to this study is given. 2008-8019 5 v. 2 no. 2014 181 197 http://www.journal-aquaticscience.com/article_73386_cf41c0ab7bb52257bd73591908f6b1bd.pdf Fouzia Nhari author Mohamed Sbaa author Jeanl Luc Vase author Mohamed Fekhaoui author Mohammed El Morhit author text article 2014 eng Leachate from municipalities’ landfills represents a potential health risk to ecosystems in generally and human populations in particularly. This study which was taken during year from 2006 to 2007 was focused to study the physicochemical evolution of the leachate from the technical enfouissment center of the Berkane city (eastern of Morocco). The analyses of the sampled leachate revealed strong content of biodegradable organic matter (BOD = 264.38 mg/l), mineral matter (conductivity = 23.33 ms/cm) and of suspended matter (SM = 38.38 mg/l). Contents in nitrate (NO3 = 53.96 mg/l) were also revealed. The long-term monitoring of the evolution of physicochemical parameters in polluted leachate, on how environmental conditions change over time, could then lead to models useful in the prediction of natural attenuation in aquifers. 2008-8019 5 v. 2 no. 2014 198 207 http://www.journal-aquaticscience.com/article_73486_897c924a1a5763957043f3374922cc1c.pdf Milena Bušova author Vojtěch Špičak author Roman Osička author text article 2014 eng Welfare issues for livestock at farms have received more and more attention in recent years. Attention focuses mainly on farms raising cattle, pigs, sheep, horses, goats or gallinaceous poultry. From the welfare point of view, the same attention is paid to fish farms whose purpose, i.e. production of fish meat, makes them comparable to livestock factory farms. Fish and fish meat hold an irreplaceable position among foods of animal origin. The aim of this study was to gather information on fish welfare in Rybnikarstvi Pohorelice, as a major producer of freshwater fish in the Czech Republic. 2008-8019 5 v. 2 no. 2014 208 214 http://www.journal-aquaticscience.com/article_73487_f94f18eb815e9c844ca6de8df5e6d499.pdf