ORIGINAL_ARTICLE http://www.journal-aquaticscience.com/article_70895_6fdb818a429fa01c60b16a742ba600ff.pdf 2016-06-06T11:23:20 2020-07-14T11:23:20 56 57 Alireza Asem true 1 AUTHOR Amin Eimanifar true 2 AUTHOR Mohammad Reza Mohammad Golizad true 3 AUTHOR

ORIGINAL_ARTICLE In the present study, water hyacinth, Eichhornia crassipes leaf is attempt to be used as protein source in Siamese Gourami, Trichogaster pectoralis farming. Preliminary study revealed that E. crassipes leaf possess high protein content (12.1±1.81%). Therefore, further study was carried out to evaluate the potential of the plant as alternative protein source for Siamese Gourami farming.There were five treatments based on different percentage of combination of E. crassipes leaf and fish meal namelyT1 (20% of E. crassipes leaf + 80% fish meal), T2 (40% of E. crassipes leaf + 60% fish meal), T3 (60% of E. crassipes leaf + 40% fish meal), T4 (80% of E. crassipes leaf + 20% fish meal) and T5 (100% of E. crassipes leaf) with three replicates for each treatment. The control group of fish was fed with fish meal. The feeding trial is continuing until four weeks and the liver of fish of each treatment is subjected to histology study in order to evaluate the toxicity level of E. crassipes leaf against fish that received treatment. The results of the present study revealed that treatment T1 showed the best performance in term of growth rate and had significance difference (p < 0.05) compared to other groups. Furthermore, T1 treatment performed promising result in palatability and toxicity experiment reflect the huge potential of the plant as protein source in Siamese Gourami farming. http://www.journal-aquaticscience.com/article_70892_8b61f93746bbd782a02f0deadd2de09e.pdf 2016-06-06T11:23:20 2020-07-14T11:23:20 58 62 <i>Trichogaster pectoralis</i> water hyacinth <i>Eichhornia crassipes</i> nutrition Seong Wei Lee true 1 AUTHOR Mat Rani Farid true 2 AUTHOR Wee Wendy true 3 AUTHOR Abdul Kari Zulhisyam true 4 AUTHOR

ORIGINAL_ARTICLE Freshwater mollusks are among the most diverse and imperiled groups of animals around the world experiencing severe population decline. This study reports the population characteristics of Batissa violacea (Lamarck, 1818) - an indigenous non-marine edible freshwater clam, in relation to the ecological condition in the largest river ecosystem in the Philippines. The B. violacea has been rated as a threatened species requiring urgent special attention for its conservation. The sampling stations were established along the downstream area of Cagayan River where the B. violacea are found covering three municipalities in the province of Cagayan. The mean density of B. violacea in Cagayan River is 1.80/m2 with a range of 0.12-11.12 clam /m2. The abundance of B. violacea in Cagayan River were found to be determined by the condition of water quality and soil substrate particularly in terms of soil pH (r=0.560; P<0.01), water pH (r=0.380; P<0.05) and total suspended solids (r=0.256; P<0.01). These results could be utilized in the formulation of the general management plan of a sanctuary established in Lallo, Cagayan, as well as the implementation of planned interconnected network of sanctuaries across the Cagayan River, to ensure a successful stock enhancement of B. violacea in Cagayan River. http://www.journal-aquaticscience.com/article_70893_6e226eb5081ab2a1f72786680bcc6c99.pdf 2016-06-06T11:23:20 2020-07-14T11:23:20 63 72 <i>Batissa violacea</i> freshwater clam Cagayan River population density Aeron Donato Mayor true 1 AUTHOR Rico C. Ancog true 2 AUTHOR Rafael D. Guerrero true 3 AUTHOR Ma Vivien C. Camacho true 4 AUTHOR

ORIGINAL_ARTICLE In the present study, sublethal toxic effects of crude oil on the biochemical and hematological parameters of Beluga (Huso huso) were studied. Two hundred juvenile Beluga (120±30g) were supplied by Rajaei fish farm in Mazandaran Province, Iran. Specimens were exposed to the sublethal concentrations of crude oil, including 0, 0.218, 0.327 and 0.436 ppm (equals to control group, 2, 3 and 4 times more than the concentration in Caspian Sea water respectively). Hematological and biochemical parameters were measured once a week for 9 weeks (63 days) of exposure. Results showed the White Blood Cell (WBC), Red Blood Cell (RBC), Hematocrit (Ht) and Hemoglobin (Hb) decreased and Mean Corpuscular Volume (MCV) and Mean Corpuscular Hemoglobin (MCH) were mainly significantly higher in fish exposed to crude oil concentrations compared to control group. A biphasic response was observed in their value throughout the study period. Results of the leukocyte numbers showed that, eosinophil numbers increased, while lymphocyte numbers decrease in comparison to the control group and during the experiments. Monocyte numbers showed no significant differences (except 49th and 63th day) while the neutrophil numbers were higher in treatment groups than the control. Biochemical parameters showed an increase in serum glucose and other parameters including total protein, Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP) and Lactate Dehydrogenase (LDH) activities were decreased in treatment groups and mainly during the experiments. The alterations of these parameters can be used as suitable biomarkers in monitoring of crude oil pollution in the aquatic environment and to protect aquatic life. http://www.journal-aquaticscience.com/article_70891_3dd15deeeb2fa166c4236b5d9cb44318.pdf 2016-06-06T11:23:20 2020-07-14T11:23:20 73 86 Biomarker Blood Great sturgeon Immunology Oil Pollution Hossein Ali Khoshbavar Rostam true 1 AUTHOR Mehdi Soltani true 2 AUTHOR

ORIGINAL_ARTICLE The experiment was designed to study the effects of different protein sources on reproductive performance and offspring quality of Macrobrachium rosenbergii in a 180-day feeding trial. Each tank was stocked with four females and one male (wet weight: 21-35g). Five isonitrogeneous (40%) and isolipidic (10%) diets were formulated to contain either fishmeal (FM), shrimp meal (SHM), squid meal (SQM), bivalve meal (BVM) or a mixture of SHM, SQM and BVM (MIX). The prawns were fed at 3% of body weight, three times daily (0800h, 1200h and 1700h). Prawn fed with diet MIX attained the highest fecundity (1449.52±64.15 egg/g female) followed by BVM (1308.53±40.41 egg/g female), SQM (1085.73±62.92 egg/g female), FM (924.84±67.75 egg/g female) and SHM (875.74±28.14 egg/g female). The fecundity of prawn fed diets MIX and BVM was significantly higher (P<0.05) than those fed SQM, FM and SHM diets. The largest egg diameter was also found in MIX diet (0.567±0.005mm) and this value was significantly larger (P<0.05) than the FM diets. In general, survival rate of larvae in all diets is very high ranging from 95.06% to 99.71%. The present study revealed that formulated diet based on shrimp, squid and bivalve meal mixture can be successfully used in the breeding program of M. rosenbergii. http://www.journal-aquaticscience.com/article_70897_c5a3e459c220ef411a92bfcedd965dc4.pdf 2016-06-06T11:23:20 2020-07-14T11:23:20 87 94 Protein reproductive performance <i>Macrobrachium rosenbergii</i> Nik Nurhusna Nik Sin true 1 AUTHOR Annita Yong Seok Kian true 2 AUTHOR Rossita Shapawi true 3 AUTHOR

ORIGINAL_ARTICLE Seminal quality evaluation is essential in aquaculture to increase reproductive efficiency in commercial species or for the introduction and population of those species that have been affected by anthropogenic damages. Sperm quality is related to volume, concentration, viability, motility and morphology. Without viable spermatozoa, there would not be any egg fertilization and embryos production, so it is of great importance to have and develop methodologies for its evaluation. Therefore, the aim of this review is to analyze and compare different methodologies that help seminal quality evaluation in teleost. http://www.journal-aquaticscience.com/article_70902_ad3fe276efa1522a0916fcc5750f36d8.pdf 2016-06-06T11:23:20 2020-07-14T11:23:20 95 106 Quality sperm concentration viability motility morphology Jesús Dámaso Bustamante-González true 1 AUTHOR Mariela González-Rentería true 2 AUTHOR Martha Rodríguez-Gutiérrez true 3 AUTHOR Araceli Cortés-García true 4 AUTHOR Alejandro Ávalos-Rodríguez true 5 AUTHOR

ORIGINAL_ARTICLE Holothuria scabra, commonly known as sandfish has a high market demand, especially in South East Asian countries such as Malaysia, Philippines and Vietnam. This species that is very popular among the Chinese is served as luxorius delicacy because it has high protein and medicinal properties. The increasing demand for sandfish has led to the over-exploitation worldwide. Hatchery production enables continuous seed production of H. scabra throughout the year by artificial spawning. Fifty healthy broodstocks collected from Kampung Baru-Baru, Tuaran Sabah (6° 18’ 18.1656” N, 116° 17’43.1052” E) were acclimatized in Universiti Malaysia Sabah (UMS) hatchery for 2 weeks prior to spawning induction. The broodstocks were fed twice a day with ground Sargassum sp. and Enhalus sp. The combination of 3 spawning induction methods: thermal shock, desiccation and algal bath were applied. Total fertilized eggs obtained were approximately 700,000, with a 9 % hatching rate. The larval rearing was carried out in high density polyethylene (HDPE) tank filled with filtered and UV treated seawater (29-30°C). The larvae were fed twice daily with Nannochloropsis sp. at a specific feeding rate. The egg and larval development were recorded daily. The duration for each stages of larval development was as followed: Gastrula (24 hours after fertilization), Early auricularia (Day 2), Mid auricularia (Day 4), Late auricularia (Day 6), Early doliolaria (Day 21), Late doliolaria (Day 24), Pentactula (Day 26). http://www.journal-aquaticscience.com/article_70899_077f51368ad9fe271320fba143fb1873.pdf 2016-06-06T11:23:20 2020-07-14T11:23:20 107 110 <i>Holothuria scabra</i> sandfish larval development spawning induction Noor Adzlina Abidin true 1 AUTHOR Sitti Raehanah Muhamad Shaleh true 2 AUTHOR Faihanna Ching true 3 AUTHOR Rafidah Othman true 4 AUTHOR Mabel Manjaji-Matsumoto true 5 AUTHOR Shigeharu Senoo true 6 AUTHOR Saleem Mustafa true 7 AUTHOR

ORIGINAL_ARTICLE The objective of this study was to analyze the impact of desiccation of a freshwater lake on the biomass of the submersed aquatic macrophytes M. spicatum and P. pectinatus. To this end, the biomasses in temporary and permanent lakes were compared for each submersed macrophyte. Differences in biomass of M. spicatum were significant between season (P<0.001) and lake x season interaction (P<0.05), but not significant between lake (P=0.114). On the other hand differences in biomass of P. pectinatus were significant between lake (P<0.001) and lake x season interaction (P<0.001). Significant differences were not found between season. http://www.journal-aquaticscience.com/article_70904_7cee36621710ccdfd89435126f42032b.pdf 2016-06-06T11:23:20 2020-07-14T11:23:20 111 117 temporary lake permanent lake growth rate aquatic plants submersed macrophyte rooted aquatic plant Hanife Özbay true 1 AUTHOR