Int. J. of Aquatic Science 2008-8019 11 1 2020 05 01 Feeding and nutrients requirement of Sultan fish, Leptobarbus hoevenii: A review 3 28 EN Hsein-Loong Au davidau940903@gmail.com Leong-Seng Lim 0000-0002-0404-1083 leongsen@ums.edu.my Thumronk Amornsakun thumronk.a@psu.ac.th Sharifah Rahmah sharifah.rahmah@umt.edu.my Hon Jung Liew honjung@umt.edu.my Poramat Musikarun mporamat@gmail.com Pornpanom Promkaew pornpanomp@hotmail.com Wen Jye Mok Gunzo Kawamura prof.gunzo@gmail.com Annita Seok-Kian Yong annitay@ums.edu.my Rossita Shapawi rossita@ums.edu.my Saleem Mustafa saleem@ums.edu.my Sultan fish (Leptobarbus hoevenii) is a commercially important freshwater fish with high potential for aquaculture production in the Southeast Asian countries, including Malaysia. Many studies have been focused on its nutrition and trophic biology but the feeding practices in the farming have not yet been reviewed. This paper reviews on nutritional management of L. hoevenii broodstock, larvae and juveniles in culture systems. In general, there are feeding guidelines developed for the L. hoevenii farming but they are not fully supported with the scientific studies. Knowledge on the larval developmental biology is lacking to fully understand the feeding ecology of this fish. In addition, there is a paucity of data on requirements of various major nutrients in L. hoevenii at different life stages. Further studies on these topics are essentially required to improve the feeding practices in the farming of L. hoevenii. Hoven’s carp,Jelawat,aquaculture,nutrition,growth http://www.journal-aquaticscience.com/article_98960.html http://www.journal-aquaticscience.com/article_98960_2797fa1d32183452bb3b7d0cc61c5f31.pdf

Int. J. of Aquatic Science 2008-8019 11 1 2020 05 01 The Efficiency of Fibrolytic Enzymes to Improving the Nutritive Quality of Local Raw Materials for Aquafeed 29 37 EN Sittichai Hatachote csnstc@ku.ac.th Kednapat Sriphairoj Orapin Jintasataporn Srinoy Chumkam A study was undertaken to evaluate the effects of supplementation fibrolytic enzymes on digestibility to improving the nutritional quality of local raw materials (soybean meal, rice bran, cassava pulp, cassava skin) for aquafeed. The objectives of the study were to improve the quality of cassava pulp and cassava skin from industrial cassava starch production by fibrolytic enzymes to obtain more sugar that can be the energy source in aquatic animals feed.The factorial experimental design (4X3) was conducted to study 4 types of raw materials (soybean meal, rice bran, cassava pulp, cassava skin) and 3 types of enzymes (xylanase at 24,000 U/kg, cellulase at 10 U/kg and mannanase at 750 U/kg). The result found that total sugars (glucose, xylose, maltose, mannose) from in vitro digestion from rice bran digestion was the highest (6.477 ± 107 mg/g) and significant differences from other raw materials (P < 0.05). However, total sugars of cassava pulp (0.900 ± 0.031 mg/g) and cassava skin (0.543 ± 0.013 mg/g) was not significantly difference from soybean meal (1.459 ± 0.054 mg/g) (P > 0.05). In soybean meal, rice bran and cassava pulp all three enzymes showed no significant differences (P > 0.05) in digestibility efficiency interestingly, cellulase exhibited significantly better digestibility with cassava skin. Total sugar from Cellulase, Xylanase and Mannanase were 0.484 ± 0.215, 0.128 ± 0.025 and 0.287 ± 0.055 respectively. Mannanase enzyme had the ability to digest fiber and released sugar from cassava pulp better than cellulase enzyme and xylanase enzyme, respectively. fibrolytic enzymes,digestibility,local raw materials,aquafeed http://www.journal-aquaticscience.com/article_101006.html http://www.journal-aquaticscience.com/article_101006_c220128ec28f3bac89bbc1e4491d0091.pdf

Int. J. of Aquatic Science 2008-8019 11 1 2020 05 01 Updated systemic account of skate fauna observed in landing at the Karachi Fish Harbour, Pakistan 38 44 EN Rabiya Nasir rabiya_zoologist@yahoo.com Nuzhat Afsar nuzhatafsar259@hotmail.com This paper presents the update and a brief account of indigenous skate fauna landed in the Karachi fish Harbour (KFH) which is the country’s largest landing site (24.8491° N, 66.9761° E) situated at West Wharf, Karachi. During field observations on catches at the Karachi Fish Harbour (KFH) a total of ten (10) species of skates were found during March 2014 to April 2019. Identified species are Glaucostegus cemiculus, G. granulatus, Rhina ancylostoma, Rhinobatos horkelii, R. punctifer, R. annandalei, R. halavi, R. obtusus, R. typus and Rhynchobatos djiddensis. Among these comparatively Glaucostegus granulatus was the species found constantly and in larger quantity. Thirty seven (37) males and twenty eight (28) females of this species were observed. Skates,Karachi Fishing Harbour,East coast,Pakistan http://www.journal-aquaticscience.com/article_98961.html http://www.journal-aquaticscience.com/article_98961_9d177ac767966f52dcba1f87202de535.pdf