Int. J. of Aquatic Science 2008-8019 7 2 2016 06 06 Urmia Lake with the names of Shaha-va-Tele and Shah-Tele 56 57 70895 EN Alireza Asem Amin Eimanifar Mohammad Reza Mohammad Golizad Journal Article 2018 10 07

Int. J. of Aquatic Science 2008-8019 7 2 2016 06 06 Water hyacinth, Eichhornia crassipes (Mart.), leaf as an Alternative Protein Source for Siamese Gourami, Trichogaster pectoralis 58 62 70892 EN Seong Wei Lee Mat Rani Farid Wee Wendy Abdul Kari Zulhisyam Journal Article 1970 01 01 In the present study, water hyacinth, Eichhornia crassipes leaf is attempt to be used as protein source in Siamese Gourami, Trichogaster pectoralis farming. Preliminary study revealed that E. crassipes leaf possess high protein content (12.1±1.81%). Therefore, further study was carried out to evaluate the potential of the plant as alternative protein source for Siamese Gourami farming.There were five treatments based on different percentage of combination of E. crassipes leaf and fish meal namelyT1 (20% of E. crassipes leaf + 80% fish meal), T2 (40% of E. crassipes leaf + 60% fish meal), T3 (60% of E. crassipes leaf + 40% fish meal), T4 (80% of E. crassipes leaf + 20% fish meal) and T5 (100% of E. crassipes leaf) with three replicates for each treatment. The control group of fish was fed with fish meal. The feeding trial is continuing until four weeks and the liver of fish of each treatment is subjected to histology study in order to evaluate the toxicity level of E. crassipes leaf against fish that received treatment. The results of the present study revealed that treatment T1 showed the best performance in term of growth rate and had significance difference (p < 0.05) compared to other groups. Furthermore, T1 treatment performed promising result in palatability and toxicity experiment reflect the huge potential of the plant as protein source in Siamese Gourami farming.

Int. J. of Aquatic Science 2008-8019 7 2 2016 06 06 Environmental factors influencing population density of freshwater clam Batissa violacea (Bivalvia) (Lamarck, 1818) in Cagayan River, Northern Philippines 63 72 70893 EN Aeron Donato Mayor Rico C. Ancog Rafael D. Guerrero Ma Vivien C. Camacho Journal Article 1970 01 01 Freshwater mollusks are among the most diverse and imperiled groups of animals around the world experiencing severe population decline. This study reports the population characteristics of Batissa violacea (Lamarck, 1818) - an indigenous non-marine edible freshwater clam, in relation to the ecological condition in the largest river ecosystem in the Philippines. The B. violacea has been rated as a threatened species requiring urgent special attention for its conservation. The sampling stations were established along the downstream area of Cagayan River where the B. violacea are found covering three municipalities in the province of Cagayan. The mean density of B. violacea in Cagayan River is 1.80/m2 with a range of 0.12-11.12 clam /m2. The abundance of B. violacea in Cagayan River were found to be determined by the condition of water quality and soil substrate particularly in terms of soil pH (r=0.560; P<0.01), water pH (r=0.380; P<0.05) and total suspended solids (r=0.256; P<0.01). These results could be utilized in the formulation of the general management plan of a sanctuary established in Lallo, Cagayan, as well as the implementation of planned interconnected network of sanctuaries across the Cagayan River, to ensure a successful stock enhancement of B. violacea in Cagayan River.

Int. J. of Aquatic Science 2008-8019 7 2 2016 06 06 The effect of chronic crude oil exposure on some hematological and biochemical parameters of juvenile Beluga (Huso huso Linnaeus, 1758) 73 86 70891 EN Hossein Ali Khoshbavar Rostam Mehdi Soltani Journal Article 1970 01 01 In the present study, sublethal toxic effects of crude oil on the biochemical and hematological parameters of Beluga (Huso huso) were studied. Two hundred juvenile Beluga (120±30g) were supplied by Rajaei fish farm in Mazandaran Province, Iran. Specimens were exposed to the sublethal concentrations of crude oil, including 0, 0.218, 0.327 and 0.436 ppm (equals to control group, 2, 3 and 4 times more than the concentration in Caspian Sea water respectively). Hematological and biochemical parameters were measured once a week for 9 weeks (63 days) of exposure. Results showed the White Blood Cell (WBC), Red Blood Cell (RBC), Hematocrit (Ht) and Hemoglobin (Hb) decreased and Mean Corpuscular Volume (MCV) and Mean Corpuscular Hemoglobin (MCH) were mainly significantly higher in fish exposed to crude oil concentrations compared to control group. A biphasic response was observed in their value throughout the study period. Results of the leukocyte numbers showed that, eosinophil numbers increased, while lymphocyte numbers decrease in comparison to the control group and during the experiments. Monocyte numbers showed no significant differences (except 49th and 63th day) while the neutrophil numbers were higher in treatment groups than the control. Biochemical parameters showed an increase in serum glucose and other parameters including total protein, Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP) and Lactate Dehydrogenase (LDH) activities were decreased in treatment groups and mainly during the experiments. The alterations of these parameters can be used as suitable biomarkers in monitoring of crude oil pollution in the aquatic environment and to protect aquatic life.

Int. J. of Aquatic Science 2008-8019 7 2 2016 06 06 Effects of different protein sources in the broodstock diet on reproductive performance of giant freshwater prawn (Macrobrachium rosenbergii) 87 94 70897 EN Nik Nurhusna Nik Sin Annita Yong Seok Kian Rossita Shapawi Journal Article 1970 01 01 The experiment was designed to study the effects of different protein sources on reproductive performance and offspring quality of Macrobrachium rosenbergii in a 180-day feeding trial. Each tank was stocked with four females and one male (wet weight: 21-35g). Five isonitrogeneous (40%) and isolipidic (10%) diets were formulated to contain either fishmeal (FM), shrimp meal (SHM), squid meal (SQM), bivalve meal (BVM) or a mixture of SHM, SQM and BVM (MIX). The prawns were fed at 3% of body weight, three times daily (0800h, 1200h and 1700h). Prawn fed with diet MIX attained the highest fecundity (1449.52±64.15 egg/g female) followed by BVM (1308.53±40.41 egg/g female), SQM (1085.73±62.92 egg/g female), FM (924.84±67.75 egg/g female) and SHM (875.74±28.14 egg/g female). The fecundity of prawn fed diets MIX and BVM was significantly higher (P<0.05) than those fed SQM, FM and SHM diets. The largest egg diameter was also found in MIX diet (0.567±0.005mm) and this value was significantly larger (P<0.05) than the FM diets. In general, survival rate of larvae in all diets is very high ranging from 95.06% to 99.71%. The present study revealed that formulated diet based on shrimp, squid and bivalve meal mixture can be successfully used in the breeding program of M. rosenbergii.

Int. J. of Aquatic Science 2008-8019 7 2 2016 06 06 Methodologies for spermatic evaluation in teleost 95 106 70902 EN Jesús Dámaso Bustamante-González Mariela González-Rentería Martha Rodríguez-Gutiérrez Araceli Cortés-García Alejandro Ávalos-Rodríguez Journal Article 1970 01 01 Seminal quality evaluation is essential in aquaculture to increase reproductive efficiency in commercial species or for the introduction and population of those species that have been affected by anthropogenic damages. Sperm quality is related to volume, concentration, viability, motility and morphology. Without viable spermatozoa, there would not be any egg fertilization and embryos production, so it is of great importance to have and develop methodologies for its evaluation. Therefore, the aim of this review is to analyze and compare different methodologies that help seminal quality evaluation in teleost.

Int. J. of Aquatic Science 2008-8019 7 2 2016 06 06 Egg and Larval Development of Induced Spawned Sandfish (Holothuria scabra) in Hatchery 107 110 70899 EN Noor Adzlina Abidin Sitti Raehanah Muhamad Shaleh Faihanna Ching Rafidah Othman Mabel Manjaji-Matsumoto Shigeharu Senoo Saleem Mustafa Journal Article 1970 01 01 Holothuria scabra, commonly known as sandfish has a high market demand, especially in South East Asian countries such as Malaysia, Philippines and Vietnam. This species that is very popular among the Chinese is served as luxorius delicacy because it has high protein and medicinal properties. The increasing demand for sandfish has led to the over-exploitation worldwide. Hatchery production enables continuous seed production of H. scabra throughout the year by artificial spawning. Fifty healthy broodstocks collected from Kampung Baru-Baru, Tuaran Sabah (6° 18’ 18.1656” N, 116° 17’43.1052” E) were acclimatized in Universiti Malaysia Sabah (UMS) hatchery for 2 weeks prior to spawning induction. The broodstocks were fed twice a day with ground Sargassum sp. and Enhalus sp. The combination of 3 spawning induction methods: thermal shock, desiccation and algal bath were applied. Total fertilized eggs obtained were approximately 700,000, with a 9 % hatching rate. The larval rearing was carried out in high density polyethylene (HDPE) tank filled with filtered and UV treated seawater (29-30°C). The larvae were fed twice daily with Nannochloropsis sp. at a specific feeding rate. The egg and larval development were recorded daily. The duration for each stages of larval development was as followed: Gastrula (24 hours after fertilization), Early auricularia (Day 2), Mid auricularia (Day 4), Late auricularia (Day 6), Early doliolaria (Day 21), Late doliolaria (Day 24), Pentactula (Day 26).

Int. J. of Aquatic Science 2008-8019 7 2 2016 06 06 Comparative study of the biomass of submersed aquatic macrophytes in a temporary and permanent freshwater lake in Turkey 111 117 70904 EN Hanife Özbay Journal Article 1970 01 01 The objective of this study was to analyze the impact of desiccation of a freshwater lake on the biomass of the submersed aquatic macrophytes M. spicatum and P. pectinatus. To this end, the biomasses in temporary and permanent lakes were compared for each submersed macrophyte. Differences in biomass of M. spicatum were significant between season (P<0.001) and lake x season interaction (P<0.05), but not significant between lake (P=0.114). On the other hand differences in biomass of P. pectinatus were significant between lake (P<0.001) and lake x season interaction (P<0.001). Significant differences were not found between season.