DNA Barcoding depicts cryptic diversity within Barilius bendelisis (Cypriniformes: Cyprinidae) from India

Document Type : Primary Research paper


1 Tufanganj College, Coochbehar, West Bengal, 736160 India

2 National Bureau of Fish Genetic Resources, Lucknow, Uttar Pradesh, 226 002 India

3 Coochbehar Panchanan Barma University, Coochbehar, West Bengal, 736101 India

4 Department of Bioscience, Barkatullah University, Bhopal, Madhya Pradesh, 462026

5 Department of Environmental Science, Barkatullah University, Bhopal, Madhya Pradesh, 462026

6 Centre for DNA Taxonomy, MSD, Zoological Survey of India, Kolkata, West Bengal, 700053 India


Members of the genus Barilius are known for their economic values. However, due to human activities and natural calamities, Barilius bendelisis faces massive population loss, especially in eastern India. We applied both morphology and molecular approaches to identify Barilius bendelisis from transboundary river Torsa, Raidak-1 and Mansai (Jaldhaka), West Bengal, India. Further, we compared genetic divergence of Barilius bendelisis from other members of the genus Barilius available in India. The Bayesian (BA) phylogeny clearly distinguishes all the studied species with reciprocal monophyletic criteria and represents multiple clades within Barilius bendelisis, indicating cryptic diversity and probable occurrence of allopatric speciation within India.