DNA Cleavage and Molecular Docking studies using Green Synthesized Annona muricata silver nanoparticles

Document Type : Primary Research paper


1 Research Scholar, Department of Chemisty, Annai Velankanni College, Tholayavatta, Kanyakumari District – 629157, Affiliated to Manonmaniam Sundaranar Univeristy, Tirunelveli – 627012

2 Associate Professor, Department of Chemistry, Annai Velankanni College, Tholayavattam, Kanyakumari District – 629157


Annona muricata has been attributed with numerous health benefits including antidiabetic, antimicrobial, etc. The aim of this study was to carry out DNA cleavage of pUC18 plasmid DNA and molecular docking against targeted protein. The DNA cleavage study was performed to know whether the isolated extract has the ability to cleave the DNA. Once the DNA was cleaved into fragments it may also lead to the death of the microbes containing the DNA which can also use as the antimicrobial agent in the drug formulation process. The molecular docking were performed to study the interaction between the molecule and the protein by which the drug interaction with the body can be known by In-Silico approach. For the studies, green synthesis of silver nanoparticles using leaf, fruit and seed of Annona muricata. Then with the sample produced the DNA cleavage studies were done with the agarose gel electrophoresis made using ethidium bromide to view the DNA under the UV light. The molecular docking was studied between alpha-d-glucopyranoside and 3VF6. The DNA cleavage studies revealed that the complete cleavage of the pUC18 plasmid DNA. In-silico Molecular docking was performed to analyse the interaction between the bioactive compound alpha-d-glucopyranoside present in Annona muricate and the targeted protein 3VF6.