Physiological Evaluation Of Someoxidative Stressbiomarkers And Enzymatic Endogenous Antioxidantamong Acute Cholecystitis Patients

Document Type : Primary Research paper


1 Department of Biology, College of Science

2 Department of General Surgery,College of Medicine University of Wasit, AL- Kut City, Wasit Province, Iraq


The current study was conducted to estimate the physiological role of enzymatic endogenous antioxidants and some cellular oxidiative stress biomarkers in acute cholecystitis patients (AC). The sample of present study consist of (150 participants) male and female including four ages groups (≤ 30, 31-45, 46-60, and ≥ 60 years) and divided into two groups: the first was the control groups of the healthy (50 participants) without acute cholecystitis and other diseases and the second was the groups of (AC) patients (100 participants) who recommended by specialist physician for laparoscopic cholecystectomy.The biochemical tests were performed for allserum blood samples taken from both study participants, including the biochemical tests for estimation of oxidiative stress biomarkers (malondialdehyde (MDA) and total oxidant status (TOS)), and some enzymatic endogenous antioxidant (catalase, glutathione peroxidase1 (GPX1), and superoxide dismutase1 (SOD1)). The serum biochemical assay for (AC) patients indicated for a clearly significant increased in theconcentrations of oxidiative stress biomarkers (MDA and TOS) in compression to control subjects. In contrast, biochemical analysis for (AC) patientsrecorded significantly decreased in the concentrations of serum enzymatic endogenous antioxidant (Catalase, SOD1, GPX1) as compared with control volunteers.It was concluded from current study data, that the oxidative stress disturbances was one of the most common causes of acute cholecystitis infection represented by highly production of reactive oxygen species (ROS), in term of functional cellular response for serum enzymatic endogenous antioxidants.