Determination of In-Vitro Antioxident And In-Vitro Antidiabetic Activity of Active Fraction From Aegle Marmelos Leaves Extract

Document Type : Primary Research paper


1 Bhabha Pharmacy Research Institute, Bhabha University, Bhopal, (M.P.)- India

2 IITM (Department of Pharmacy), IES University, Bhopal,(M.P.)- India Corresponding Author


Aegle marmelos leaves has been used as a remedy for gastrointestinal infections, antidiarrheal, anti-inflammatory and antioxidant activity of human beings. Extraction was obtained using methanol and fractionation in column chromatography with different solvent followed with: beginning with chloroform, ethyl acetate, methanol and water. The invitro antioxidant activity, total flavonoid content and Total polyphenol content seven fraction of Aegle Marmelos leaf extract was determine by using spectrophotometric method. The all fraction obtained from extract were based on solvent concentration for seven fractions. The total antioxidant capacity of the fractions was measured in mg/ml of ascorbic acid, with a range of 35 to 132 mg/ml, total flavonoid content of the fractions was expressed of quercetin equivalent (QE) ranging between 47.5 – 83.5 mg/ml and total phenolic content of the fractions were expressed of Gallic acid equivalent (GAE) ranging between 96.3 – 181.5. All the fractions, it was observed that water fraction having better antioxidant property, so the it was preferred for invitro diabetic activity. The Percentage inhibition of Acarbose was found to be 33.23 μg/ml and fraction of ethanolic extract of Aegle marmelos percentage inhibition was to be 156.84 μg/ml.