In Vitro Antibacterial, Antioxidant And Time-Kill Kinetics Of Cow Ark With Plectranthus Amboinicus Extract Against Human Pathogens

Document Type : Primary Research paper

Author

Pharmacognosy lab, Department of Animal Health and Management, Alagappa university, Karaikudi 630003.

Abstract

The antimicrobial and antioxidant activity of the cow ark with Plectranthus Amboinicus extract on multidrug resistant bacteria and fungi caused by human pathogens. The present study investigated to antibacterial activity of cow ark with Plectranthus Amboinicus (CAM) extract was evaluated by agar well diffusion method against microbial strains. CAM was more susceptible for influential the antimicrobial activity Protein leakage assay and Time Kill assay. The CAM inhibited the growth of bacteria and fungi with high-levels of antibiotic-resistance, such as Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, Klebsiella Pneumoniae, Aspergillus niger, Candida albicans. The CAM has antioxidant potentials to reduce the oxidative stress by using DPPH radical Scavenging Assay. The results showed that the protein leakages of bacteria and fungi are correlated to the concentration of CAM. Time-kill kinetics profiles of CAM showed highly significant bactericidal and fungicidal activities. Our results proposed that the CAM bioactive compounds, which may be useful against antibiotic-resistant bacteria and Fungi

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