Document Type : Primary Research paper
Department of Microbiology, Smt. Chandibai Himathmal Mansukhani College, Ulhasnagar – 03, Maharashtra, India.
Department of Dairy Microbiology, National Dairy Research Institute, Karnal, Haryana.
Department of Microbiology, Smt. Chandibai Himathmal Mansukhani College, Ulhasnagar – 03, Maharashtra, India
Mahim creek a salt-water body located in Mumbai district Maharashtra was explored for isolation of Prodigiosin producing bacteria. A total of 38 pigmented isolates were obtained of which 15.78% were red pigmented. Two isolates namely MK 12 and MK 23 successfully qualified for prodigiosin production. Acetone extraction was more effective with a yield of 23.6ug/10gm and 47.30ug/ml for MK12 and MK23, respectively. Based on isolates morphological and biochemical characteristics they were identified as Serratia rubidaea and Serratia marcescens. MIC for antibacterial activity against standard pathogens for MK 12 and MK 23 was in the range of 2ug/ml to 3ugml and 2ug/ml to 4ug/ml. While for both the extracts MIC for antifungal activity was 4ug/ml. Both the extracts were effective in dyeing cotton fabric in presence and absence of mordants (FeSO4 and Al2(SO4)3). Simultaneous mordanting with FeSO4 was the more effective method for dyeing cotton fabrics for both the extracts. Stability studies showed percent loss in the range from 3% to 12%, with dye being most stable under alkaline conditions. Dyeing was most effective for wool and nylon fabrics as detected on the multifibre strip.