Semen evaluation in Chirostoma jordani (Woolman, 1894) and Chirostoma humboldtianum (Valenciennes, 1835), Mexican native species (Atheriniformes: Atherinopsidae)

Document Type: Primary Research paper

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Abstract

The knowledge of the reproductive physiology of fish of commercial interest is fundamental to optimize reproduction in wildlife or in captivity, so this research aims to determine and compare the seminal quality of Chirostoma jordani and Chirostoma humboldtianum. A sample of 20 specimens of each species from the state of Tlaxcala and Mexico City were obtained, respectively, from which the semen was obtained by slightly pressing the abdominal region in the operculum-caudal direction, collected with a micropipette of 100 μL and placed in microcentrifuge tubes. According to the results, the mean volume of semen was 2.73 ± 1.0 μL for C. jordani and 2.47 ± 1.8 μL in C. humboldtianum, while the mean sperm concentration was 9.24 ± 4.32 x 106 spermatozoa mL-1 and 15.58 ± 13.81 x 106 spermatozoa μL-1 respectively, with 95.10 ± 4.90 % and 92.10 ± 1.89 % of live cells respectively, and average motility of 437.85 ± 90.37 s and 549.40 ± 31.80 s respectively. Finding significant differences in the motility time between both species. Information that contributes to the knowledge of the reproductive biology of C. jordani and C. humboldtianum to improve their reproduction in captivity or wildlife.

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