Isolation and Characterization of Probiotic Bacteria from the Gastrointestinal Tract of Pond-cultured Litopenaeus vannamei in Tuaran, Sabah

Document Type : Primary Research paper



Sabah has the highest annual whiteleg shrimp (Litopenaeus vannamei) aquaculture production in Malaysia. However, disease infection is one of the big problems to whiteleg shrimp aquaculture. One of the promising control strategies is theusing of probiotics. Currently, the study on probiotic in Sabah is very limited. Therefore, this study aimed to isolate, characterize and identify potential probiotic bacteria from the gut ofpond-cultured whiteleg shrimp in Tuaran, Sabah. Eight out of a total of twenty one isolatesfrom TSA, Rogosa and MRS agarwere screened out and designated as GS4, GS11, GS12, GS14, GS15, WS1, WS3 and WS5. However, four isolates (GS11, GS12, GS15 and WS5) had probiotic potential for shrimp culture. They are identified as Shewanella sp. (WS5), Bacillus thuringiensis (GS11), Lactobacillus plantarum (GS12), and B. cereus (GS15). Among the four selected isolates, GS15 has the highest probiotic potential due to positive antagonistic activity against pathogenic V. harveyi. Further research such as in vivo assay still needs to be conducted to test the probiotic potential of the four selected isolates from the gut of collected L. vannamei samples. Besides being used to support disease management of L. vannamei aquaculture in Sabah, all four isolates might help aquaculture of other penaeid shrimp or non-penaeid shrimp species.