Pharmacological studies (Analgesic and Hemolytic) on the cone snail venom Conus coronatus Gmelin, 1791

Document Type : Primary Research paper



The venom of the cone snails has a rich source of novel peptides with pharmaceutical activity. The aim of this study was to investigate the hemolytic, and analgesic effects of Conus coronatus venom. Samples were collected from Qeshm Island, Persian Gulf. The venom ducts were isolated and kept on ice then homogenized. The mixture was centrifuged and the supernatant was considered as a crude venom. The hemolytic activity was performed on human red blood cell and purification was carried out by using gel filtration chromatography on Sephadex G-25. The analgesic effect was evaluated via intraperitoneally (IP) injection in mice. Finally, the molecular weight of the analgesic fractions was determined by using Tricine-SDS-PAGE. Results showed that the crude venom exhibited no hemolytic activity on human erythrocytes and the purify fraction number C2 with dose 0/5 mg/kg showed the best analgesic activity in both acute and inflammatory pain and exhibited a dose-dependent analgesic effect (P<0.05) containing peptides with the molecular weight less than 6.5 kDa. The venom of the C. coronatus from the Persian Gulf contains an analgesic component for relieving acute and inflammatory pain with a small size and no toxicity which can lead to finding a new analgesic drug.