Document Type : Primary Research paper
Authors
1
Department of Microbiology, Acharya Nagarjuna University, Nagarjuna nagar, Guntur, India
2
Department of Microbiology, Acharya Nagarjuna University, Nagarjuna nagar, Guntur, Andhra Pradesh - 522510, India.
3
FED, Lakireddy Bali Reddy College of Engineering, Mylavaram, A.P., India
Abstract
It turns out that there is a tremendous amount of wealth below the surface of the ocean waiting to be discovered. The ocean environment is capable of solving many unsolved questions and is also home to an undiscovered hoard of mysteries. Seawater collected from Suryalanka marine sediments in Guntur, Andhra Pradesh, India was used to extract coastal soil samples. Selected bacterial strains were isolated using the spread plate technique, the bacteria with biological activity were screened using antibacterial activity, and the 16S rRNA sequencing was utilised to identify the bacteria. Metabolite which is biologically active was created in a defined setting (incubation, temperature, pH, Nacl concentration, and carbon amino acid and nitrogen sources). TLC was used to purify crude extract, which was then eluted with hexane to separate it on a column. Spectrally characterised purified fraction was examined for antibacterial and anticancer properties. Out of the 23 isolates, isolate AVSC3 exhibits a noticeable inhibitory activity against test organisms. This was established through a characterization of the test organism, Bacillus subtilis AVSC3, with a GenBank accession number of GEO:M21745. MN386243. To produce bioactive metabolites, an ideal incubation time is 60 hours, 35°C, pH 7, 0.5% NaCl, and 1% glucose and peptone. The antibacterial property of the pure fraction was found to be hexadecanoic acid, with a molecular formula of C16H32O2, which has spectral characteristics characteristic of this molecule. 14.2 mm-long Coli followed by Staphylococcus The 13.7-mm-diameter aureus, the 9-mm-diameter M.luteus, the 8.1-mm-diameter S.pyogenes, the 4.6-mm-diameter P. vulgaris, and the 4.4-mm-diameter S.typhi all demonstrated significant antimicrobial activity against MCF-7 cell lines, with the strongest effect observed at 25μg.
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