Molecular Isolation of Bacteria Pathogenic from Lung Disorder Patients

Document Type : Primary Research paper


Biotechnology college/Agricultural University of Al-Qadisiyah


This study aimed to determine the bacterial species colonizing the Lung Disorder Patients in Central Al-Dewaniyah, Iraq on a microbiological and molecular level. Nasal swab samples were obtained from 20 samples attendants in the period of time extending from June to November 2020 in Al-Dewaniyah city, Iraq. Microbiological identification techniques were utilized to identify the bacterial species isolated. Molecular identification techniques based on PCR. Two bacterial species were isolated from both the nasal cavity and the oropharynx including, S. pnemonaie and H. influenza. The results of the current study showed after collecting 20 samples from patients suffering from respiratory symptoms, where samples were taken by cotton swab from the upper respiratory tract and cultured in the laboratory, where we found that 10 samples are positive S. pnemonaie and 7 positive samples to H. influenzae. The virulence genes for each of the two diagnosed bacteria were also examined, and it was found that there were 6 positive samples for each of the virulence genes for the bacteria S. pnemonaie. The results of the virulence genes for bacteria were H. influenzae 3 positive for a gene HMW2 and 4 positive for a gene hifA as shown in Table 1 and fig.1,2,3,4,5 and 6.